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Custom Antisera
CEDARLANE® manufactures Complement, Lympholyte® cell separation media, cell purification Immunocolumns and a wide range of Antibodies. Also, CEDARLANE® continues to offer our Custom Antibody Services.
Cedarlane-manufactured Reagents
Our line of Cedarlane-manufactured reagents.
- Animal Red Blood Cell Reagents
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DescriptionCedarlane’s Purified Anti-Rat Crry monoclonal antibody (Clone: 5I2) is a rat-specific membrane complement regulator that can inhibit both antibody-induced classical pathway and alternative pathway complement activation. Crry plays a more dominant role than DAF in regulating the alternative pathway of complement and in preventing spontaneous complement damage of rat erythrocytes, whereas DAF and Crry are both expressed and are equally effective in preventing antibody-induced runaway complement activation on rat erythrocytes.
This antibody is suitable for use in flow cytometry6 and immunohistochemistry (acetone-fixed frozen sections).3,4,5,6______________________________________________________________________________________________________Flow Cytometry Analysis
Wistar rat splenocytes (left) and bone marrow (right) were stained with anti-Crry (clone: 5I2) (filled histogram) or mouse IgG2a isotype control (open histogram).______________________________________________________________________________________________________Immunohistochemical Analysis
CL064AP [Anti-Rat Crry, Purified (Clone 5I2)] staining of rat spleen (Frozen section) at a concentration of 0.1 μg/mL (Magnification approximately 150X).
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Product Name:
Anti-Rat Crry, Purified (Clone 5I2) (mouse IgG1)
Code:
CL064AP
Supplier:
Cedarlane
Size:
250ug
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Product Name:
Anti-Rat Crry, Biotin (Clone 5I2) (mouse IgG1)
Code:
CL064B
Supplier:
Cedarlane
Size:
100ug
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Unit Price: |
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Product Name:
Anti-Rat Crry, FITC (Clone 5I2) (mouse IgG1)
Code:
CL064F
Supplier:
Cedarlane
Size:
100ug
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Unit Price: |
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References
1) Molina, H., Miwa, et al. 2002. Complement-mediated clearance of erythrocytes: mechanism and delineation of the regulatory roles of Crry and DAF. The American Society of Hematology
2) Ohta, R., et al. 1999. Characteration of Mouse DAF on Trasfectant Cells Using Monclonal Antibodies Which Recognize Different Epitopes. Nagoya City University School of Medicine and The Scripps Research Institute.
3) Nishikawa, K., et al. 1996. Local inflammation caused by a monoclonal antibody that blocks the function of the rat membrane inhibitor of C3 convertase. The Journal of Immunology. 156: 1182-1188.
4) Nomura, A., et al. 1995. Tubulointerstitial injury induced in rats by a monoclonal antibody that inhibits function of a membrane inhibitor of complement. J. Clin. Invest. Vol. 96. 2348-2356.
5) Matsuo, S., et al. 1994. In vivo effects of monoclonal antibodies that functionally inhibit complement regulatory proteins in rats. J. Exp. Med. Vol. 180. 1619-1627.
6) Nishikage, H., et al. 1995. The role of a complement regulatory protein in rat mesangial glomerulonephritis. J. Am. Soc. Nephrol. 6: 234-242.
Custom Antibody Services
CEDARLANE® manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® continues to offer our Custom Antibody Services and increased demand has lead to the continued expansion of our facility. We understand the varied nature of custom work and encourage you to call our qualified staff to discuss the details of your project. Please inquire about other services not listed. Be assured that we at CEDARLANE® are dedicated to ensuring the confidentiality of all projects we discuss.
