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Cedarlane › Reagents › Cedarlane Manufactured › Tools for Flow Cytometry › Flow Cytometry Antibodies › Mouse › Erythroid Cells (Ly-76) › TER-119

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CEDARLANE® manufactures Complement, Lympholyte® cell separation media, cell purification Immunocolumns and a wide range of Antibodies. Also, CEDARLANE® continues to offer our Custom Antibody Services.

  

Cedarlane-manufactured Reagents

Our line of Cedarlane-manufactured reagents.

TER-119

  • Description

    Cedarlane’s purified anti-mouse erythroid cell (Ly-76) mAb is selectively reactive with both fetal and adult erythroid cells. This monoclonal antibody (clone: TER119) is specific for cells at stages from early proerythroblast to mature erythrocytes. 

    TER119 is reported to react with 20-25% of bone marrow cells and 2-3% of spleen cells but not with thymocytes or lymph node cells. In fetal haematopoietic tissues, 30-40% of day 10 yolk sac cells, 80-90% of day 14 fetal liver cells and 40-50% of newborn liver cells were reactive with CL8926AP. TER119+ cells in adult bone marrow expressed significant levels of CD45 but not myeloid (Mac-1, Gr-1) or B cell (B220) markers. 

    This mAb immunoprecipitated protein bands with molecular weights of 110 kDa, 60 kDa, 52 kDa and 32 kDa from erythrocyte membrane whereas only a 52 kDa band was detected by TER119 in Western Blot analysis. It has been determined that the TER119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself. Also the antigen is only expressed on normal erythroid cells but not on erythroleukaemia cells.

    This product is suitable for use in flow cytometry. It has also been reported to work in Western Blot, immunoprecipitation and immunohistochemistry (frozen and paraffin).
    ______________________________________________________________________________________________________

    Flow Cytometry Analysis
    Balb/c whole blood (left) or bone marrow (right) were stained with anti-Ly 76 (clone: TER119) (filled histogram) or rat IgG2b isotype control (open histogram).
       
Product Name: Anti-Mouse Erythroid Cell (Ly 76), Alexa Fluor®488 (Clone TER119) (rat IgG2b)
Code:
CL8926AF4
Supplier:
Cedarlane
Size:
100 ug
Unit Price:
$369.00 CDN
Product Name: Anti-Mouse Erythroid Cell (Ly 76), Purified (Clone TER119) (rat IgG2b)
Code:
CL8926AP
Supplier:
Cedarlane
Size:
250ug
Unit Price:
$283.00 CDN
Product Name: Anti-Mouse Erythroid Cell (Ly 76), Biotin (Clone TER119) (rat IgG2b)
Code:
CL8926B
Supplier:
Cedarlane
Size:
100ug
Unit Price:
$165.00 CDN
Product Name: Anti-Mouse Erythroid Cell (Ly 76), Biotin (Clone TER119) (rat IgG2b)
Code:
CL8926B-3
Supplier:
Cedarlane
Size:
300ug
Unit Price:
$453.00 CDN
Product Name: Anti-Mouse Erythroid Cell (Ly 76), FITC (Clone TER119) (rat IgG2b)
Code:
CL8926F
Supplier:
Cedarlane
Size:
100ug
Unit Price:
$188.00 CDN
Product Name: Anti-Mouse Erythroid Cell (Ly 76), FITC (Clone TER119) (rat IgG2b)
Code:
CL8926F-3
Supplier:
Cedarlane
Size:
300ug
Unit Price:
$429.00 CDN
Product Name: Anti-Mouse Erythroid Cell (Ly 76), Low Endotoxin (Clone TER119) (Rat IgG2b)
Code:
CL8926LE
Supplier:
Cedarlane
Size:
500 ug
Unit Price:
$511.00 CDN
Product Name: Anti-Mouse Erythroid Cell (Ly 76), No Azide (Clone TER119) (rat IgG2b)
Code:
CL8926NA
Supplier:
Cedarlane
Size:
1 mg
Unit Price:
$609.00 CDN
Product Name: Anti-Mouse Erythroid Cell (Ly 76), PE (Clone TER119) (rat IgG2b)
Code:
CL8926PE
Supplier:
Cedarlane
Size:
50ug
Unit Price:
$193.00 CDN
Product Name: Anti-Mouse Erythroid Cell (Ly 76), PE (Clone TER119) (rat IgG2b)
Code:
CL8926PE-3
Supplier:
Cedarlane
Size:
300ug
Unit Price:
$749.00 CDN

  • References

    1)  Kina, T., Ikuta, K., Takayama, E., Wada, K., Majumdar, A. S., Weissman, I. L. and  Katsura, Y.  (2000) British  Journal of Haematology 109, 280-287.

    2)  Ikuta, K., Kina, T., MacNeil, I., Uchida, N., Peault, B., Chien, Y. and Weissman, I. L.  (1990) Cell, Vol. 62, 863-874.

    3)  Randall, T. D. and Weissman, I. L. (1998) Stem Cells 16, 38-48.

    4)  Suwabe, N., Takahashi, S., Nakano, T. and Yamamoto, M. (1998) Blood, Vol 92, No 11, 4108-4118.

    5)  Mukouyama, Y., Chiba, N., Mucenski, M. L. Satake, M., Miyajima, A., Hara, T. and Watanabe, T. (1999)  Current Biology 9, 833-836.

    6)  Yanai, N., Matsui, N., Matsuda, K.-I., Furusawa, T., Okubo, T., Nakazawa, T., Ishibashi, K., Nawa, K. and Obinata, M. (1999)  Experimental Hematology 27, 1087-1096.

    7)  Plant, A., and  J. Tobias (2002) J. Bone Miner. Res. 17:782-790.

    8)  Hartner, J.C., Schmittwolf, C., Kispert, A., Muller, A. M., Higuchi, M., and P. Seeburg (2004) J. Biol. Chem.  279 (6): 4894-4902.

Custom Antibody Services

CEDARLANE® manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® continues to offer our Custom Antibody Services and increased demand has lead to the continued expansion of our facility. We understand the varied nature of custom work and encourage you to call our qualified staff to discuss the details of your project. Please inquire about other services not listed. Be assured that we at CEDARLANE® are dedicated to ensuring the confidentiality of all projects we discuss.



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