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Anti-Rat CD45RA/B Alexa Fluor®488 (Clone OX-33) (mouse IgG1)
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Product Code:
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CL033AF4 |
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Supplier Name:
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Cedarlane |
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Size:
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100 ug |
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Data Sheet:
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View Data Sheet |
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Categories:
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OX-33 |
Additional Product Details
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Applications:
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F, C, P |
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Clone:
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OX-33 |
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Format:
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Alexa Fluor488 |
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Isotype:
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Mouse IgG1 |
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Specificity:
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CD45RA/B |
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Host:
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Mouse |
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Conjugate:
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Alexa Fluor488 |
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Species Reactivity:
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Rat |
$369.00 CDN
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DescriptionThe rat CD45 antigen (Leukocyte Common Antigen (L-CA)) has been shown to exist in different forms on different lymphoid cell types. Rat CD45 on thymocytes shows one main band at 180 kDa; T cells show 4 bands at 180, 190, 200 and 220 kDa. Clone OX-33 detects a sub-fraction of the 240 kDa rat CD45 band found only on B lymphocytes. This monoclonal antibody is of particular use in the investigation of the molecular and antigenic heterogeneity of rat L-CA, especially when used in conjunction with other OX antibodies. Four mouse monoclonal antibodies (MRC OX-1, 28, 29 and 30) react with all molecular forms of L-CA and fall into two sets that are non-competitive in binding to L-CA (MRC OX-1, 28, 29 and 30). The antigenic determinants seen by all these antibodies are lost when L-CA is reduced and alkylated. Three antibodies (MRC OX-22, 31, and 32) react selectively with B cells, T cytotoxic cells and about two thirds of T helper cells. OX-22 and OX-31 compete for binding but are non-competitive with OX-32. All of these antibodies bind to a sub-fraction of the 190, 200 and 220 kDa forms of T cell L-CA but not at all to the 180 kDa form of T cells or thymocytes.
This clone works in flow cytometry and with frozen and paraffin sections (2).______________________________________________________________________________________________________Flow Cytometry Analysis
Wistar rat splenocytes stained with anti-CD45RA/B (clone: OX-33) (filled histogram) or mouse IgG1, k isotype control (open histogram).
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References
1) Woolett, G.R., Barclay, A.N. Paklavec, M., and A.G. Williams (1985). Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes. Eur. J. Immunol. 15: 168-173.
2) Whiteland, J.L et al (1995). Immunohistochemical detection of T cell subsets and other leukocytes in paraffin embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320.
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