Canada
United States
International
M1/70.15
-
DescriptionCedarlane’s anti-mouse CD11b (Mac-1; Ly 40) monoclonal antibody detects the 170 kDa a subunit of Mac-1 which mediates adhesion to ICAM-1 (CD54) and C3bi. Mac-1 is expressed on granulocytes, macrophages, natural killer cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 is up-regulated on neutrophils after activation. This particular clone blocks cell adherence and C3bi binding but does not block cell mediated lysis (1,2,3,4,5,6).Applications include flow cytometry, in vitro and in vivo blocking, immunohistochemistry for both paraffin embedded and acetone-fixed frozen sections 1-20 µg/ml (7). Also useful for immunoprecipitation and western blotting.______________________________________________________________________________________________________Flow Cytometry Analysis
C3H/HE mouse peritoneal macrophages (left) and bone marrow (right) were stained with anti-CD11b (clone: M1/70.15) (filled histogram) or rat IgG2b isotype control (open histogram).______________________________________________________________________________________________________Immunofluorescence Analysis
Purified Anti-Mouse CD11b (CL8941AP) staining of paraformaldehyde-fixed paraffin-embedded mouse spleen sections at 400x magnification with DAPI counterstain. (Rat anti-mouse CD11b mAb (CL8941AP) 1:500; Alexa Fluor 488 Goat anti-rat 1:500 (Molecular Probes) and Normal Goat Serum (blocking))
- 1
- 2
- 1
- 2
-
References
1. Sanchez-Madrid, F., P. Simon, S. Thompson, et al. 1983. Mapping of antigenic and functional epitopes on the α and β subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J. Exp. Med. 158:586-602.
2. Springer, T., G. Galfre, D.S. Secher, et al. 1978 Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8: 539 - 551.
3. Springer, T., G. Galfre, D.S. Secher, et al. 1979. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur. J. Immunol. 9:301 - 306.
4. Vignali, D.A., et al. 1990. Antibody-Dependent killing of Schistosoma mansoni Schistosomula in vitro by Starch-elicited murine macrophages. J. Immunol. 144: 4030 -40378.
5. Kantor, A., et al. 1992. Differential development of progenitor activity for three B-cell lineages. Proc. Natl. Acad. Sci. U.S.A. 89: 3320 - 3324.
6. Jutial, M.A., et al. 1988. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma. J. Immunol. 18: 1819 - 1826.
7. Whiteland, J.L. et al. (1995). Immunohistochemical detection of T cell subsets and other leukocytes in paraffin embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. Volume 43, pp 313-320.
Custom Antibody Services
CEDARLANE® manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® continues to offer our Custom Antibody Services and increased demand has lead to the continued expansion of our facility. We understand the varied nature of custom work and encourage you to call our qualified staff to discuss the details of your project. Please inquire about other services not listed. Be assured that we at CEDARLANE® are dedicated to ensuring the confidentiality of all projects we discuss.
