30% OFF ZYMOBIOMICS PRODUCTS
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The Only Comprehensive Solution for Microbiomics and Metagenomics!
Overcome bias in your microbiomics workflow by using ZymoBIOMICS™ Microbial Community Standards to compare different DNA extraction protocols. DNA samples were profiled by 16S rRNA gene targeted sequencing.
Assess GC-Bias in Shotgun Metagenomics
Library preparation for shotgun metagenomic sequencing was performed in two different ways using Supplier A and an in-house method. Shotgun sequencing was performed on MiSeq with paired-end sequencing (2x150 bp). Raw reads were mapped to the 10 microbial genomes to evaluate the potential effect of GC content on sequencing coverage. Normalized coverage was calculated by normalization with the average sequencing coverage of each genome.
Track PCR Chimera in 16S rRNA Gene Sequencing
PCR chimera increases with PCR cycle number in the library preparation process of 16S rRNA gene targeted sequencing. 20 ng ZymoBIOMICS™ Microbial Community Standard was used a template. The PCR reaction was performed with ZymoTaq master mix and with primers that target v3-4 region of 16S rRNA gene. Chimera rate in percentage was determined with Uchime and using the 16S rRNA gene of the 8 bacterial strains in the standard as reference.
This promotion applies to the following products:
|D4300||ZymoBIOMICS™ DNA Mini Kit||50 Preps|
|D4300T||ZymoBIOMICS™ DNA MiniPrep Kit||5 Preps|
|D4301||ZymoBIOMICS™ DNA Microprep Kit||50 preps|
|D4302||ZymoBIOMICS™-96 MagBead DNA Kit (Includes ZR BashingBead™ Lysis Rack)||2 x96 Preps|
|D4303||ZymoBIOMICS™-96 DNA Kit (Includes ZR BashingBead™ Lysis Rack)||2x96 Preps|
|D4304||ZymoBIOMICS™ DNA Miniprep Kit (Lysis Matrix Not Included)||50 Preps|
|D4305||ZymoBIOMICS™ DNA Microprep Kit (Lysis Matrix Not Included)||50 Preps|
|D4306||ZymoBIOMICS™-96 MagBead DNA Kit (Lysis Matrix Not Included)||2x96 preps|
|D4307||ZymoBIOMICS™ 96 DNA Kit (Lysis Matrix Not Included)||2x96 preps|
|D4308||ZymoBIOMICS™-96 MagBead DNA Kit (Includes ZR BashingBead™ Lysis Tubes)||2x96 preps|
|D4309||ZymoBIOMICS™-96 DNA Kit (Includes ZR BashingBead™ Lysis Tubes)||2x96 preps|
|R2001||ZymoBIOMICS™ RNA Mini Kit||50 Preps|
|R2002||ZymoBIOMICS™ DNA/RNA Mini Kit||50 Preps|
|D6300||ZymoBIOMICS™ Microbial Community Standard||10 Preps|
|D6305||ZymoBIOMICS™ Microbial Community DNA Standard||200 ng|
|D6306||ZymoBIOMICS™ Microbial Community DNA Standard||2000 ng|
|E2056(ZY)||ZymoBIOMICS™ PCR Premix||50 Reactions|
|E2057||ZymoBIOMICS™ PCR Premix||200 Reactions|
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Note: This offer is not valid in conjunction with contract discounts or any other special pricing. Void where prohibited.