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Anti-Human Tamm Horsfall Ascites (Clone 10.32) (mouse IgG2b)

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Miscellaneous Antibodies 10.32

Additional Product Details

E, P, C
Mouse IgG2b
Tamm Horsfall (Uromucoid)
Species Reactivity:
$402.00 CDN

  • Description

    Cedarlane anti-human Tamm-Horsfall protein (THP) is a monoclonal antibody which reacts with an epitope of the urinary mucoprotein. Tamm-Horsfall protein is a glycoprotein of approximately 80 kDa containing up to 25% carbohydrate by weight. It exists as a high molecular weight polymer in urine and approximately 50 mg of the THP are excreted per day by adult humans. THP is produced by the epithelial cells lining the thick ascending loop of Henle and distal convoluted tubules of the kidney. THP is also the primary constituent of renal tubular casts and THP aggregation has been implicated as a factor in the pathogenesis of acute renal failure by causing tubular obstruction. Antibody to THP is present in the serum of patients with pyelonephritis and reflus uropathy.

    CL1032AP can be used in indirect immunosorbent enzyme assays or in immunohistopathology for staining of frozen sections and formalin fixed, paraffin embedded tissues by indirect immunofluorescence or immunoperoxidase. It can also be used to purify THP by binding to a solid phase. This monoclonal antibody has shown good localization to the distal tubule and collecting duct as well as to large deposits of Tamm-Horsfall protein which has clogged peri-pelvic veins. This antibody also works with canine tissue.

    Immunohistochemical Analysis

    IHC staining of formalin-fixed, paraffin-embedded human tissue (kidney) using Purified Anti-Human Tamm Horsfall (CL1032AP) at a dilution of 20 μg/mL.

  • References

    1) Bhagavan, B.S., R.E. Wenk and D. Dutta. 1979. Pathways of Urinary Backflow in Obstructive Uropathy. Human Pathology. 10: 669-683.

    2) McKenzie, J.K., and E.G. McQueen. 1969. Immunofluorescent localization or Tamm-Horsfall mucoprotein in human kidney. J. Clin. Path. 22:334-339.

    3) Zager, R.A., R.S. Cotran & J.R. Hoiyer. 1978. Pathologic Localization of Tamm-Horsfall Protein in Interstitial Deposits in Renal Disease. Lab Invest. 38: 52-57.

    4) Nanus, D.M., et al. 1989. Transformation of human kidney proximal tubule cells by ras-containing retroviruses. Implications for tumor progression. J. Exp. Med. 69: 953-972.

    5) Nagashima, Y. et al. 2005. Renal oncocytosis. Pathology International. 55: 210-215.

    6) Howell, D.N., et al. 1991. Characterization of a novel human corneal endothelial antigen. Investigative Ophthalmology and Visual Science, 32: 2473-2482.