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Anti-human CDCrel-1, Biotin, (clone SP18), (Mouse IgG1)
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Product Code:
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CL2708B |
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Supplier Name:
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Cedarlane |
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Size:
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100ug |
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Data Sheet:
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View Data Sheet |
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Categories:
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SP18 |
Additional Product Details
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Applications:
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WB, E, F |
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Clone:
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SP18 |
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Format:
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Biotin |
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Isotype:
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Mouse IgG1 |
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Specificity:
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CDCrel-1 |
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Host:
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Mouse |
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Conjugate:
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Biotin |
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Species Reactivity:
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Human |
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DescriptionCDCrel-1/Septin 5 (Sept5) belongs to the Septin family of nucleotide binding GTPases. Septins were originally described in yeast as cell division cycle regulatory proteins involved in cytokinesis and the regulation of cytoskeletal organization. Sept5 is expressed in cells of the nervous system and is seen to associate primarily with vesicles and membranes through its interaction with the SNARE domain of syntaxin 1A. Through its interaction with syntaxin 1A Sept5 acts to inhibit exocytosis, possibly by regulating vesicle targeting and/or fusion. Recently it was shown that Sept5 is phosphorylated by cyclin-dependent kinase 5 (Cdk5) – p35 decreasing it’s binding to syntaxin 1A. This suggests that Cdk5 can modulate synaptic vesicle release by regulating the interactions between Sept5 and syntaxin 1A.______________________________________________________________________________________________________Immunofluorescence Analysis
Purified Anti-Human CDCrel-1/Septin 5 (CL2708AP) staining of paraformaldehyde-fixed paraffin-embedded human cerebellum sections at 400x magnification with DAPI counterstain. (Mouse anti-human CDCrel-1/Septin 5 (CL2708AP) 1:500; Alexa Fluor 488 Goat anti-mouse 1:500 (Molecular Probes) and Normal Goat Serum (blocking))
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References
1) Beites et al. The septin Sept5/CDCrel-1 competes with α-SNAP for binding to the SNARE complex. Biochem. J. 385, 347-353, 2005.
2) Honer, WG et al. Cingulate cortex synaptic terminal proteins and neural cell adhesion molecule in schizophrenia. Neuroscience. 78(1), 99-110, 1997.
3) Honer, WG et al. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex. Brain Res. 609(1-2), 9-20, 1993.
4) Honer, WG et al. Monoclonal antibodies to study the brain in schizophrenia. Brain Res. 500(1-2), 379-83, 1989.
5) Lang, T. and Jahn R. Core proteins of the secretory machinery. Handb. Exp. Pharmacol. (184): 107-27, 2008.
6) Taniguchi, M et al. Phosphorylation of adult type Sept5 (CDCrel-1) by cyclin- dependent kinase 5 inhibits interaction with syntaxin-1. J. Biol. Chem. 282(11): 7869-76. 2007.
7) Ungermann,C. and Langosch, D. Functions of SNAREs in intracellular membrane fusion and lipid bilayer mixing J. Cell Science. 118, 3819-3828, 2005.
