Canada
United States
International
Anti-Mouse gamma/delta TCR, No Azide (Clone GL3) (hamster IgG)
|
Product Code:
|
CL7201NA |
|
Supplier Name:
|
Cedarlane |
|
Size:
|
1mg |
|
Data Sheet:
|
View Data Sheet |
|
Categories:
|
GL3 |
Additional Product Details
|
Applications:
|
F, C |
|
Clone:
|
GL3 |
|
Format:
|
No Azide |
|
Isotype:
|
Hamster IgG |
|
Specificity:
|
Gamma/Delta TCR |
|
Host:
|
Hamster |
|
Conjugate:
|
Unconjugated |
|
Species Reactivity:
|
Mouse |
-
DescriptionCedarlane's anti-mouse gdT cell receptor monoclonal antibody reacts with the surface on all gdTCR bearing cells and does not react with receptors on ab TCR positive cells. It is thought that this clone may be specific for a determinant present on Cd 7. The gd T cell receptors are present on murine CD4-CD8- thymocytes, peripheral T cells, intestinal CD8+ intraepithelial lymphocytes and Thy 1+ dendritic epidermal cells in the skin 1.Use of this antibody in conjunction with an anti-CD3 monoclonal antibody (Cedarlane's anti-CD3e Monoclonal Antibody CL7202, clone 145-2C11) allows for accurate measurements of the mutually exclusive sub-populations of gd TCR and abTCR bearing T cells. Cedarlane's anti mouse gd TCR monoclonal antibody has also been used successfully for the characterization of murine intraepithelial lymphocytes.This clone is reported to work with frozen sections6.______________________________________________________________________________________________________Flow Cytometry Analysis
C3H/He mouse splenic T-cells were stained with anti-gamma/delta TCR (clone: GL3) (filled histogram) or Armenian hamster IgG isotype control (open histogram).
-
References
1) Sunderland,C.A., McMaster, W.R., and A.F. Williams. (1979) Eur. J.Immunol. 9, 155-159. Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.
2) Brown,W.R.A., Barclay,A.N., Sunderland,C.A. and A.F. Williams. (1981) Nature. 289, 1164-1177. Identification of a glycoprotein-like molecule at the cell surface of rat thymocytes.
3) Standring,R. and A.F. Williams. (1978) Biochim.Biophys. Acta. 508, 85-96. Glycoproteins and antigens of membranes prepared from rat thymocytes after lysis by shearing or with the detergent Tween-40.
4) Brown,W.R.A. and A.F. Williams. (1982) Immunology. 46, 713-726. Lymphocyte cell surface glycoproteins which bind to soybean and peanut lectins.
5) Woollet,G.R., Barclay,A.N., Puklavec,M. and A.F. Williams. (1985) Eur. J. Immunol. 15, 168-173. Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.
6) Brouard, S., et al. (1999) J. of Immunol. 162, 3367-3377. T Cell repertoire alterations of vascularized xenografts.
