Adult Mouse Hypothalamus Cell Line mHypoA-2/4

Adult Mouse Hypothalamic Cell Lines

This product line is based on a proprietary platform technology which has enabled the creation of a series of immortalized hypothalamic neurons. Using CNTF (ciliary neurotrophic factor) to trigger neurogenesis, the cells were immortalized from two month old male (CLU170-CLU194) or two month old female (CLU450-CLU472) C57Bl/6 mice hypothalamic primary cultures by retroviral transfer of SV40 T-Ag. These cell lines have been found to express an ever expanding array of neuropeptides, enzymatic markers and biologically active receptors.

As such, these cell lines enable accurate in-vitro assays for use in the discovery, development and validation of new therapeutics targeted to central-nervous system diseases and disorders, including obesity, stress, and metabolic disorders, amongst others.

Further details regarding the cells can be found in the following papers:

• Endocrinology (2011) by Dhillon et al.
• FASEB J (2009) by Belsham et al.

The datasheet for this product can be downloaded here.

Download the Adult Mouse Hypothalamic Cell Line Screen Profile

For information regarding additional markers not listed or to find out how these cell lines can easily be screened for additional peptides, receptors, and markers of interest, please contact us for more details.

For detailed information regarding cell line expression please click the Catalogue # highlighted in the table below:

Immunofluorescence Analysis

Anti-Ghrelin (CL8818AP) staining using goat anti-rabbit Alexa-594 secondary antibody on Adult Mouse Hypothalamus Cell Line mHypoA-2/12 cells (CLU177) imaged under confocal microscope with the 63X oil objective; nuclei were counterstained with DAPI (blue).

Anti-PGP 9.5 (CL7755AP) staining using goat anti-rabbit Alexa-488 secondary antibody on Adult Mouse Hypothalamus Cell Line mHypoA-2/12 cells (CLU177) imaged under confocal microscope with the 40X oil objective; nuclei were counterstained with DAPI (blue).