Hypothalamic

Mouse and Rat Hypothalamic Cell Lines

Please select a category above for detailed information.

Embryonic Mouse Hypothalamic Cell Line Screen Profile

Embryonic Rat Hypothalamic Cell Line Screen Profile

Adult Mouse Hypothalamic Cell Line Screen Profile

mHypoA-GnRH/GFP Cell Line Screen Profile

Imaging of Embryonic and Adult Hypothalamic Cell Lines

Examples of hypothalamic cell lines, both embryonic and adult, are illustrated within this figure.

(A-D) The embryonic mHypoE-46, -29/2, -38 and -43/5 were imaged using phase contrast microscopy. These four lines were used in studies described in the energy homeostasis section and the mHypoE-38 line was also used in studies described in the reproduction section.

(E-I) The adult mHypoA-2/22, -2/1, -2/3 and -2/5 were imaged using confocal differential interference contrast microscopy.

(J) The embryonic mHypoE-38 neurons were imaged using fluorescent confocal microscopy after immunocytochemical analysis with anti-ghrelin sera (green); nuclei were counterstained with propidium iodide (red).

(K) The adult mHypoA-2/12 neurons were imaged using fluorescent microscopy after immunocytochemical analysis with an antibody against NPY (green); nuclei were counterstained with DAPI (blue).

(L and M) The mHypoE-36/1 neurons were imaged using DAB staining during immunocytochemical analysis with antibodies against neurofilament (NF) and neurotensin (NT).

Reference: C.M. Mayer et al., Hypothalamic cell lines to investigate neuroendocrine control mechanisms, Front. Neuroendocrinol. (2009), doi:10.1016/j.yfrne.2009.03.005

For information regarding additional markers not listed or to find out how these cell lines can easily be screened for additional peptides, receptors, and markers of interest, please contact us for more details.