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DNA Damage EIA Kit
The DetectX® DNA Damage Immunoassay Kit is designed to quantitatively measure DNA and RNA oxidized guanosine species. The assay detects all three oxidized guanine species; 8-hydroxy-2’-deoxyguanosine from DNA, 8-hydroxyguanosine from RNA and 8-hydroxyguanine from DNA or RNA. These species may be present in serum, plasma, saliva, urine, dried fecal samples, and tissue culture media samples.
An 8-hydroxy-2’-deoxyguanosine (8-OHdG) stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An 8-hydroxyguanosine conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a peroxidase-labeled mouse monoclonal antibody to 8-hydroxy-2’-deoxyguanosine to each well. After a 2 hour incubation the plate is washed and substrate added. The substrate reacts with the peroxidase labeled antibody that has reacted with the bound conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the 8-hydroxy-2’-deoxyguanosine in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.
- USE - Measure Oxidized Guanine molecules
- SAMPLE - Serum, Plasma, Saliva, Urine, Fecal Extracts and TCM
- SAMPLES/KIT - 38 or 230 in Duplicate
- STABILITY - Liquid 4˚C Stable Reagents
- SENSITIVITY - Measure 51 pg/mL in 2.5 Hours
Arbor Assays LLC was formed in July 2007 in Ann Arbor, Michigan by three scientists who collectively have over 50 years of experience in designing, manufacturing, analyzing and shipping FDA regulated and life science and clinical products worldwide. Two of the founders have worked at Abbott Labs Diagnostics Division, Kallestad Diagnostics, Sanofi Diagnostics, R&D Systems, and in 1992 founded Assay Designs, Inc. “ Our philosophy is to build the highest quality detection and immunoassay products for clinically important biomolecules. ”Assay Designs is a company that has grown from 2 part-time employees to be a major worldwide supplier of immunoassay and detection kits.
Arbor Assays’ company philosophy is to build the highest quality detection and immunoassay products for clinically important biomolecules. Our primary customer focus is both pharmaceutical and clinical scientists. We build robust immunoassay and enzyme activity kits to quantitate biomolecules in biological matrices. We delight in taking on the most technically challenging assays there are to develop. The thrill of building a kit that makes someone's job easier or allows someone to measure something no one else has been able to is what drives us. And we are driven!
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