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YAML 501.4
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DescriptionCedarlane’s anti-Human CD45 monoclonal antibody specifically binds the human CD45 Leukocyte Common Antigen (T200). CD45 is a family of single-chain transmembrane glycoproteins that consists of at least four isoforms (220, 205, 190, 180 kDa). Anti-Human CD45 monoclonal antibody recognizes an epitope common to all known isoforms. CD45 is expressed on hematopoietic cell lines, but not mature red cells, and is not detected on differentiated cells of other tissues. The protein phosphatase activity of the intracellular portion of CD45 is involved in transmembrane signaling for regulation of various cellular functions. Antibody to CD45 is used to differentiate lymphomas from non-hematopoietic undifferentiated neoplasms.______________________________________________________________________________________________________Flow Cytometry Analysis
Human peripheral blood lymphocytes were stained with anti-CD45 (T200) (clone: YAML 501.4) (filled histogram) or Rat IgG2a isotype control (open histogram).______________________________________________________________________________________________________Immunofluorescence Analysis
Purified Anti-Human CD45 (T200) (CL7605AP) staining of paraformaldehyde-fixed paraffin-embedded human spleen sections at 400x magnification with DAPI counterstain. (Rat anti-human CD45 (T200) (CL7605AP) 1:500; Alexa Fluor 488 Goat anti-rat 1:500 (Molecular Probes) and Normal Goat Serum (blocking))
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Product Name:
Anti-Human (CD45 (T200)), Alexa Fluor®488 (clone YAML 501.4), (Rat IgG2a)
Code:
CL7605AF4
Supplier:
Cedarlane
Size:
100 ug
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Product Name:
Anti-Human (CD45 (T200)), Alexa Fluor®647 (clone YAML 501.4), (Rat IgG2a)
Code:
CL7605AF6
Supplier:
Cedarlane
Size:
100 ug
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Product Name:
Anti-Human (CD45 (T200)), Alexa Fluor®700 (clone YAML 501.4), (Rat IgG2a)
Code:
CL7605AF7
Supplier:
Cedarlane
Size:
100 ug
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Unit Price: |
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Product Name:
Anti-Human (CD45 (T200)), Purified (clone YAML 501.4), (Rat IgG2a)
Code:
CL7605AP
Supplier:
Cedarlane
Size:
200 ug
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Product Name:
Anti-Human (CD45 (T200)), APC (clone YAML 501.4), (Rat IgG2a)
Code:
CL7605APC
Supplier:
Cedarlane
Size:
100 ug
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Product Name:
Anti-Human (CD45 (T200)), APC-CY7 (clone YAML 501.4), (Rat IgG2a)
Code:
CL7605APC-CY7
Supplier:
Cedarlane
Size:
100 ug
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Product Name:
Anti-Human (CD45 (T200)), Biotin (clone YAML 501.4), (Rat IgG2a)
Code:
CL7605B
Supplier:
Cedarlane
Size:
100 ug
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Product Name:
Anti-Human (CD45 (T200)), FITC (clone YAML 501.4), (Rat IgG2a)
Code:
CL7605F
Supplier:
Cedarlane
Size:
100 ug
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Unit Price: |
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Product Name:
Anti-Human (CD45 (T200)), PE (clone YAML 501.4), (Rat IgG2a)
Code:
CL7605PE
Supplier:
Cedarlane
Size:
50 ug
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Unit Price: |
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Product Name:
Anti-Human (CD45 (T200)), PE-Cy7 (clone YAML 501.4), (Rat IgG2a)
Code:
CL7605PE-CY7
Supplier:
Cedarlane
Size:
100 ug
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Unit Price: |
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References
1) Aqel NM, Ball RY, Waldmann H, Mitchinson MJ. Identification of macrophages and smooth muscle cells in human atherosclerosis using monoclonal antibodies. J Pathol. 146(3):197-204, 1985.
2) Johnson P, Greenbaum L, Bottomly K, Trowbridge IS. Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies. J Exp Med. Mar 1; 169 (3):1179-84. 1989.
3) Streuli M, Hall LR, Saga Y, Schlossman SF, Saito H. Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens. J Exp Med. Nov 1; 166 (5):1548-66. 1987.
4) Hale G, Clark M, Waldmann H. Therapeutic potential of rat monoclonal antibodies: isotype specificity of antibody-dependent cell-mediated cytotoxicity with human lymphocytes. J Immunol. May; 134(5):3056-61. 1985.
Custom Antibody Services
CEDARLANE® manufactures complement, Lympholyte® cell separation media, cell purification immunocolumns, neuronal cell lines and a wide range of antibodies. CEDARLANE® continues to offer our Custom Antibody Services and increased demand has lead to the continued expansion of our facility. We understand the varied nature of custom work and encourage you to call our qualified staff to discuss the details of your project. Please inquire about other services not listed. Be assured that we at CEDARLANE® are dedicated to ensuring the confidentiality of all projects we discuss.
