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ATCC Cell Lines and Culture

  • About the American Type Culture Collection

    The American Type Culture Collection is a long-standing organization established in 1925 and dedicated to a wide range of scientific practices. As a biological resource center, ATCC authenticates microorganisms and cell lines while managing the long-term preservation and distribution of cultures for the scientific community. ATCC supports the cultures it acquires and authenticates through expert technical support, intellectual property management, and comprehensive characterization data. The American Type Culture Collection is recognized for its diverse collection of biological research solutions.

    Leading Medical Research & Biological Materials Management

    As a research organization, ATCC works to produce innovative knowledge and technology while consistently improving its function as a biological resource center. ATCC scientists develop new in vitro model systems, define new species, investigate disease biomarkers, and build valuable characterization datasets for biological materials.

    The American Type Culture Collection has an extensive microorganism collection that includes 3,000 different types of animal viruses and various strains used in vaccine development. The organization is dedicated to using its resources and experience to lead in biological reference materials, particularly through the acquisition of new content, the development of reference standards using unique assets, and the advancement of biological research through standardization and certification.

    ATCC offers specialized and custom biological resource center services, including secure deposit services for customers’ cell cultures. ATCC products and services are used nationally and internationally, primarily by researchers in academia and government organizations.

    Throughout its operations, ATCC demonstrates a strong commitment to meeting and exceeding industry standards related to ethics, intellectual property, management, and corporate responsibility. These principles support the wide range of products and services upon which the American Type Culture Collection is built.

    Product Types

    • Cells and Microorganisms
    • Culture Reagents
    • Testing and Characterization
    • Quality Control Stains
    • Nucleic Acids & Toxins

    Reporter-Labeled Strains: Powerful Tools for Microbiology Research

    Discover the reporters commonly used in microbiological research and explore the different applications of reporter-labeled strains in this ATCC white paper.

    Download the White Paper
  • ATCC Learning Center

    The ATCC Learning Center provides researchers with tangible resources for advancing and supporting their research. This includes the latest ATCC newsletters, upcoming webinar registration information, product sheets, and much more! Click here to access these valuable resources!

    ATCC® Standards Development Organization Releases Third ANSI Standard

    ATCC Standards Development Organization released its third ANSI Standard: Species‐Level Identification of Animal Cells through Mitochondrial Cytochrome c Oxidase Subunit 1 (CO1) DNA Barcodes (ANSI/ASN‐0003‐2015) for interspecies cell line identity based on sequence technology. This new standard complements the existing standard, ASN-0002- Authentication of Human Cell Lines: Standardization of STR Profiling, which is used for human cell line and tissue identification.

    Click here for more information.

    ATCC Webinars

    Explore the expanding collection of ATCC webinars to learn more about the innovative research and development being performed by thought leaders in science.Click here to access these webinars!

  • Primary Product Categories

    • Cell Lines and Microbial Cultures
    • Disease/Model
    • Tissue
    • Tumor Cell Panels
    • Human Cell Lines
    • Animal Cell Lines
    • Hybridomas
    • Reporter Cell Lines
    • Isogenic Cells
    • Neural Progenitor Cells
    • Solute Transport Carrier Cells
    • Immuno-Oncology Cell Lines
    • Microbial Panels
    • Primary Cells
    • Stem Cells
    • Organoids
    • Culture Reagents
    • Buffers and Stains
    • Media
    • Spheroids

    About ATCC

    ATCC is a private, non-profit biological resource center (BRC) and research organization focused on the acquisition, authentication, production, preservation, development, and distribution of standard reference microorganisms, cell lines, cell cultures, and other materials for life science research. As a long-established organization in the life sciences, ATCC is a reliable and recognized name that provides high-quality products and services. Cedarlane is proud to distribute ATCC’s extensive product range, with a particular emphasis on affordability and efficiency.

  • ATCC® Primary Human Hepatocytes

    ATCC Primary Human Hepatocytes

    ATCC offers high-quality primary human hepatocytes that serve as a gold standard for liver research. These cells retain many of the in vivo morphological and functional characteristics of hepatocytes, making them ideal for applications in:

    • Drug metabolism and pharmacokinetics (DMPK) studies
    • Toxicology testing and safety assessments
    • ADME (Absorption, Distribution, Metabolism, and Excretion) research
    • Disease modeling, including liver fibrosis and viral hepatitis

    ATCC hepatocytes are:

    • Characterized and quality-controlled for key biomarkers and functionality
    • Available as cryopreserved lots, ensuring convenience, reproducibility, and consistency
    • IRB-consented and ethically sourced to support compliance and research integrity

    With a comprehensive selection of cryopreserved hepatocytes from multiple donors, ATCC provides researchers with reliable, physiologically relevant models to advance translational and preclinical studies.

    EXPLORE ATCC PRIMARY HUMAN HEPATOCYTES ›

  • Introducing MicroQuant™ by ATCC: Precision in Microbial Quality Control

    Streamline your microbial QC testing with ATCC’s latest innovation, MicroQuant™. Specifically designed for pharmaceutical manufacturing, MicroQuant™ provides accurately quantitated microbial reference materials to ensure reliable, consistent, and precise results every time.

    Why Choose MicroQuant™?

    Using cutting-edge cryopreservation technology, MicroQuant™ offers stable, single-use pellets that rehydrate rapidly, delivering fast, accurate, and reproducible results. Simplify your workflow and reduce turnaround times without compromising on quality.

    ATCC MicroQuant Workflow

    Key Benefits

    • Precision Quantitation: Available in high (107-108 CFU) and low (100-1,000 CFU) titer formats, guaranteeing consistency across lots.
    • Stringent Quality Control: Stringent quality control measures help uphold biopharmaceutical production standards.
    • Single-Use Convenience: Ready-to-use pellets reduce setup time and eliminate the need for extensive microbial culture preparation.
    • Easy Storage: Stable for up to six months at 2-8°C, ensuring availability when you need it.
    • Trusted Quality: Manufactured under ISO 17034 by ATCC, ensuring top-tier quality and traceable source materials.

    Stay ahead in microbial quality control. Choose MicroQuant™ for dependable, efficient, and high-quality results.

  • ATCC Cell Products

    Organoids built for predictive relevance and translational insight

    When predictive relevance drives experimental outcomes, start with clinically annotated, patient-derived organoids built for consistent, defensible results. These genomically characterized systems support non-animal research approaches and more accurately reflect disease biology, heterogeneity, and treatment response than conventional models, helping scientists make more confident, defensible decisions in drug development and translational studies.

    A Compendium of Next-Generation Patient-Derived Models for Diverse Cancers

    ElHarouni D, et al. Nature 2026
    Unlock the full complexity of cancer with a next-generation resource of clinically annotated, patient-derived models designed to reflect real tumor biology. Co-authored by ATCC scientists, this publication demonstrates how this powerful framework is advancing precision oncology and enabling more confident, data-driven therapeutic insight.

    Organoid collection

    Organoid collection

    Organoids derived from healthy and cancerous tissues across diverse physiological systems.

    ORDER ORGANOIDS ›
    Organoid panels

    Organoid panels

    Standardized panels of patient-derived organoids from breast, pancreatic, and colorectal cancers.

    EXPLORE ORGANOID PANELS ›
    Organoid growth kits

    Organoid growth kits

    Growth kits matched to a specific organoid subtype for consistent media preparation.

    VIEW ORGANOID GROWTH KITS ›
  • Unlock Innovation with ATCC’s Trusted and Diverse Cell Models

    ATCC Cell Products

    ATCC offers the world’s largest and most diverse collection of authenticated human and animal cell lines, encompassing over 4,000 continuous cell lines categorized by species, tissue or disease type, and signaling pathway.

    Key Offerings

    • Human Cells: ATCC provides a vast array of human cell cultures essential for scientific discovery and innovation.
    • Animal Cells: The collection includes cell lines from more than 150 different species, serving as vital models for human disease research.
    • Primary Cells: Normal human primary cells that retain in vivo physiologies, with custom lots available from a diverse donor pool.
    • Immortalized Primary Cells: hTERT-immortalized primary cells exhibiting normal in vivo biologies, enhanced proliferative capabilities, and suitability for high-passage applications.
    • Stem Cells: Ethnic- and gender-diverse induced pluripotent stem cells (iPSCs) derived from normal and diseased tissues, as well as adult human mesenchymal stem cells and mouse embryonic stem cells.
    • Advanced Cell Models: Including isogenic cell lines with key oncogene mutations created using CRISPR/Cas9 technology, and luciferase-labeled cells for sensitive measurement of biological processes.
    • Human Cancer Models Initiative (HCMI): Next-generation 2-D and 3-D patient-derived in vitro cancer models, including organoids, developed in collaboration with HCMI to advance cancer research and therapeutic development.

    ATCC’s commitment to rigorous quality control and cell authentication ensures that researchers receive credible cell cultures, leading to reproducible and reliable scientific results.

  • Easy-to-Follow Culture Guides

    Culturing biological materials can be challenging at times, even for the expert culturist. With nearly a century of expertise in cell and microbial cultures, ATCC has acquired and developed a vast body of best practices to help researchers at all levels of proficiency maximize the return on their biomaterials investment. The guides below deliver that knowledge and insight in a portable, easy-to-follow format.

    Animal Cell Culture Guide
    Animal Cell Culture Guide
    Click to download
    Virology Guide
    Virology Guide
    Click to download
    Stem Cell Culture Guide
    Stem Cell Culture Guide
    Click to download
    Protistology Guide
    Protistology Guide
    Click to download
    Primary Cell Culture Guide
    Primary Cell Culture Guide
    Click to download
    Organoid Culture Guide
    Organoid Culture Guide
    Click to download
    Mycology Culture Guide
    Mycology Culture Guide
    Click to download
    Introduction to Microbiology
    Introduction to Microbiology
    Click to download
    Bacterial Culture Guide
    Bacterial Culture Guide
    Click to download
  • Thousands of Authenticated Reference Genomes: ATCC Genome Portal

    ATCC has been working toward providing authenticated whole-genome sequences from its microbial collection through the ATCC Genome Portal. This continuously expanding resource provides researchers with authenticated reference genomic data that are directly traceable to trusted ATCC biological materials.

    The ATCC Genome Portal is the only authenticated reference omics database for ATCC biomaterials.

    ATCC Genome Portal Qtr 2 Updates June 03, 2026

    • Released new datasets for 49 human and 2 organoid cell lines
    • Added genomes for 225 bacteria, 7 viruses, 16 fungi, and 2 protists
    • Published 32 genomes supporting the FDA’s GenomeTrakr initiative
      • To find these records, enter GenomeTrakr in the advanced search feature and select the Tag filter.
    • Expanded to include 4.5K epigenome and 5.3K bacterial resistome profiles

    Changes to ATCC Genome Portal Access

    As the ATCC Genome Portal continues to grow, ATCC now offers Supporting Membership opportunities to help ensure the portal’s long-term sustainability and continued expansion.

    Supporting Members receive expanded access to authenticated genome assemblies and annotations, secure REST API capabilities, Discrepancy Reports, bulk download options, and future extended features.

    Researchers without a Supporting Membership can continue searching for genomes of interest, viewing available organism and genome information, and accessing genomic data associated with eligible ATCC strains they have purchased.

    Explore Authenticated Genomic Data

    Access authenticated, reference-quality genomic data and discover the membership option that best supports your research needs.

    Explore the ATCC Genome Portal

    For questions or feedback about the ATCC Genome Portal, contact nextgen@atcc.org.

  • ATCC Toxicology Models

    Credible models for every stage of your toxicological studies

    Harmful substances can be present in pharmaceutical drugs, and the testing of these substances determines the degree of toxicity. This will often determine the fate of whether a new drug progresses through the development pipeline. It is critical that the standards and model organisms used in toxicological testing are reliable and authenticated. We can help streamline your research by providing the most authenticated, advanced, and functional models available. Let ATCC revolutionize and accelerate your toxicology studies in every phase of the research and testing process.

    Where are you in your testing?

    Modeling Screening and Characterizing

    Modeling, Screening, Characterizing

    Get started on the path to success in the preclinical arena with ATCC’s complete solution of continuous cell lines, growth reagents, and cell viability assays.

    GET STARTED ›
    Pharmacology Exploratory Toxicology

    Pharmacology, Exploratory Toxicology

    ATCC provides advanced cell models for toxicity testing, such as differentiated iPSCs and primary and hTERT-immortalized primary cells.

    EXPLORE OUR RESOURCES ›
    Preclinical Toxicology PK Metabolism

    Preclinical Toxicology PK Metabolism

    Explore our resources to discover how neurological and renal cell models can support your metabolic stability, metabolite identification, and drug-drug interaction assays.

    BROWSE PRECLINICAL RESOURCES ›

  • Reporter-labeled Cells

    Reporter-labeled Cells

    Reporter-labeled cell lines are invaluable research tools for studying gene expression and for screening cell lines. Some of the most commonly used reporter systems are those that induce a visually identifiable phenotype such as the emission of fluorescent or luminescent light or the production of a pigmented product. Fluorescent reporters exhibit a fluorescent signal upon exposure to specific wavelengths of light; luminescent reporters use a luciferase enzyme to catalyze a reaction with its substrate, luciferin, to produce visible light; and chromogenic reporters employ an enzyme label that reacts with a substrate to produce a pigmented product.

    Advantages of Reporter-labeled Cultures

    Reporter systems have an array of applications in the basic and applied sciences. In biological research, reporter systems provide a measurable and distinguishable phenotype that can be applied in the analysis of:

    • Quantification
    • Detection
    • Host-pathogen interactions
    • Drug discovery
    • Compound screening
    • Toxicity studies
    • In vivo imaging
    • Quality control
    • Pathway research
    • Differentiation studies

    Discover more at: https://www.atcc.org/cell-products/reporter-labeled-cells

  • ATCC Cell Line Land Webinar

    Enhance Your Research with the ATCC Cell Line Land Webinar

    Unlock the full potential of your research with insights from the recorded webinar "ATCC Cell Line Land: Reference-Quality RNAseq Data from Credible ATCC Cell Lines".

    In this comprehensive webinar, industry experts delve into:

    • The Importance of Credible Cell Lines: Understand why using authentic ATCC cell lines is crucial for reliable research outcomes.
    • Reliable and Authentic RNAseq Data: Learn how ATCC ensures the highest quality and reliability in their RNAseq data.
    • Practical Applications: Discover how RNAseq data can be applied across various fields of study to enhance your research.

    Watch the Webinar Recording:

    Title: ATCC Cell Line Land: Reference-Quality RNAseq Data from Credible ATCC Cell Lines
    Link: Watch the Recording

    Don't miss this opportunity to learn from the best and leverage ATCC's trusted resources to take your research to the next level. Watch the recording today!

  • Save on ATCC Products through Cedarlane
    ATCC®Genomic RNA isolated from a preparation of Severe acute respiratory syndrome-related coronavirus 2 isolate USA/MD-HP05285/2021. This product can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.


    Save on ATCC Products through Cedarlane
    This product is a whole-genome sequenced preparation of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) strain 2019-n-CoV/USA-WA1/2020 that has been inactivated by heating. This strain was originally isolated from a human case in Washington state and was deposited by the Centers for Disease Control and Prevention. This product is intended for use in molecular assays or as a process control (eg, viral RNA extraction through qPCR amplification process), and it can also be used to make a dilution series to estimate level of detection.

    Save on ATCC Products through Cedarlane
    This product is a preparation of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) strain USA/MD-HP05285/2021 (Delta variant) that has been inactivated by heating at 65°C for 30 minutes and is therefore unable to replicate. This heat-inactivated preparation is whole-genome sequenced and can be used as a process control in the development of SARS-CoV-2 molecular diagnostic tools and can also be used to make a dilution series to estimate the level of detection. Due to the dynamic nature of lineage determination using PANGO, it is recommended to confirm the provided GISAID information prior to initiation of work

    Save on ATCC Products through Cedarlane
    A vial of heat-inactivated SARS-CoV-2 variant B.1.351 (Beta). This strain was isolated from a clinical specimen in Maryland and deposited by the CDC.

  • Save on ATCC Products through Cedarlane
    ATCC®VR-3326HK is a preparation of SARS-CoV-2 strain USA/CA_CDC_5574/2020 (Alpha variant) that has been inactivated by heating. The strain was isolated in December 2020 from a nasopharyngeal swab from a human in San Diego, California, and was deposited by the Centers for Disease Control and Prevention. This heat-inactivated preparation is whole-genome sequenced and can be used as a process control in the devleopment of SARS-CoV-2 molecular diagnostic tools and can also be used to make a dilution series to estimate the level of detection.


    Save on ATCC Products through Cedarlane
    Genomic RNA isolated from a preparation of Severe acute respiratory syndrome-related coronavirus 2 strain 2019-nCoV/USA-WA1/2020. This product can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.

    Save on ATCC Products through Cedarlane
    This product is a preparation of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) strain USA/COR-22-063113/2022 (Omicron variant) that has been inactivated by heating at 65°C for 30 minutes and is therefore unable to replicate. This heat-inactivated preparation is whole-genome sequenced and can be used as a process control in the development of SARS-CoV-2 molecular diagnostic tools and can also be used to make a dilution series to estimate the level of detection.

  • Monthly Feedback Draw

    NEW Genomic DNA Control Materials for Measurement of Residual DNA - ATCC-USP Collaboration

    Analytical Reference Materials for Residual Host Cell DNA Testing

    Regulators require that manufacturers demonstrate the removal of genomic DNA from biological therapies and vaccines since it can pose a safety risk if it is not purified from the product. As biopharmaceutical innovation continues to advance, trusted reference materials and standards can help accelerate biologics development.

    ATCC and USP have come together to offer solutions that help ensure the quality of cell and gene therapies, vaccines, and recombinant proteins. The initial set of six products consists of highly characterized and pure genomic DNA (gDNA) from cell lines used in bioproduction that can be used to measure residual host cell DNA.

    These products and materials support the most common platforms used in the rapidly growing cell and gene therapy and vaccines sectors:

    • Quantitative HEK-293 Genomic DNA
    • Quantitative Sf9 Genomic DNA
    • Quantitative Vero Genomic DNA
    • Quantitative MDCK Genomic DNA
    • Quantitative MRC-5 Genomic DNA
    • Quantitative BHK-21 Genomic DNA

    Click here to learn more about these high-quality reference materials.

    For more about the ATCC-USP collaboration, visit here.

  • cGMP Mammalian Cell Banking and Biorepository

    ATCC's cGMP cell banking and biorepository services are designed to meet rigorous clinical and commercial manufacturing standards. They provide end-to-end support, including cell line authentication, sterility testing, and mycoplasma testing, ensuring the integrity and reliability of biological materials. Their state-of-the-art facilities offer secure, long-term storage with controlled environments to maintain cell bank viability and stability.

    ATCC also offers regulatory support and documentation, essential for FDA and EMA compliance. Their expert team facilitates the technical transfer of cell banks, ensuring a smooth transition to production phases. This comprehensive approach minimizes risks and accelerates the path to market.

    Key features of ATCC’s services include:

    • Development and storage of Master and Working Cell Banks with comprehensive characterization.
    • Stringent quality control measures to uphold biopharmaceutical production standards.
    • Advanced storage facilities ensuring cell bank stability.
    • Regulatory compliance support for seamless FDA and EMA approval.
  • News

    ATCC and USP Enter Strategic Collaboration to Support Global Quality Production of Biologics

    Manassas, VA, February 2023 - ATCC and USP to provide solutions and standards to help customers deliver safe and high-quality biologics to patients more rapidly. ATCC, the world’s premier biological materials management and standards organization and U.S. Pharmacopeia (USP), an independent, international, and scientific nonprofit organization, today announced a long-term, multi-year strategic collaboration to jointly provide co-branded reference materials and reference standards that will serve to advance the quality and development of biologic medicines and therapies. Read more.

    ATCC Announces Award from Leidos Biomedical Research for the Production and Distribution of Next-generation Cancer Models

    January, 2022 — ATCC, the world’s premier biological materials management and standards organization, today announced that it has been awarded a contract with Leidos Biomedical Research, Inc. (Leidos Biomed), current operator of the Frederick National Laboratory for Cancer Research, for the continued production, characterization and distribution of next-generation cancer models (NGCM). These models will be distributed solely by ATCC as part of the National Cancer Institute’s (NCI) Human Cancer Model Initiative (HCMI), an international consortium that is generating novel, next-generation, tumor-derived culture models annotated with genomic, clinical and biospecimen data. In consultation with the HCMI team at Leidos Biomed and the Center for Cancer Genomics (CCG) at NCI, the contract will add 70 additional NGCMs to HCMI’s current catalog of 182 models – all of which will be available as a community resource through its data portal. Read more.

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