The new iCELLigence system from ACEA Biosciences is a unique, impedance-based system for cell-based assays, allowing for label-free and real-time monitoring of cellular processes such as cell growth, proliferation, cytotoxicity, adhesion, morphological dynamics and modulation of barrier function.
The system is composed of an electronic analyzer that fits inside the cell culture incubator, and an iPad which runs the software and operates the entire system in a wireless mode. At the core of the system are electronic plates (E-Plates L8) that have integrated microelectrode sensors in the bottom of the wells. The presence or absence of cells sensitively and precisely affects the electronic and ionic exchange between cell culture media and the microelectrodes when an electric field is applied to the system. Changes in electrical impedance reflect the biological status of the cells, thus the system allows monitoring of time-dependent perturbation effects in a tissue culture environment, providing dynamic information and a unique approach to cell-based assays.
Real-time monitoring of cellular processes by the iCELLigence system offers distinct and important advantages over traditional end-point assays, and allows researchers to explore dynamic cellular analysis for a variety of research applications in drug development, toxicology, cancer, medical microbiology, and virology.
The RTCA iCELLigence System is composed of three main components:
- RTCA iCELLigence Analyzer
- RTCA Control Unit (iPad)
- E-Plate L8
ICELLIGENCE INSTRUMENT HIGHLIGHTS:
- iPad compatible GUI
- Intuitive workflows
- Wireless data acquisition